Skrining pada Berbagai Jenis Umbi dan Pisang sebagai Sumber Difructose Anhyride III (DFA III) Melalui Reaksi Enzimatis
نویسندگان
چکیده
Difructosa Anhyride III (DFA III) dapat diproduksi melalui reaksi enzimatis menggunakan inulin. Inulin diperoleh dari akar/umbi dan batang tanaman. Pada penelitian ini, jenis umbi-umbian pisang yang diketahui sebagai salah satu sumber inulin akan diproses untuk pembuatan DFA fructotransferase (IFTase) Nonomurae sp. Tujuan ini adalah melakukan tahapan skrining memperoleh bahan baku berbagai umbi berpotensi III. Metode digunakan yaitu fruktotransferase (IFTase). Umbi diblansing, dipotong diblender ditambahkan air panas dengan rasio 1:2. Ekstrak dihasilkan diukur secara kualitatif metoda kromatografi lapis tipis (KLT) metode spektrofotometer. Hasil menujukkan bahwa jalar mempunyai potensi besar dalam pembentukan kadar sebesar 7.03%, sedangkan tidak III, hanya memiliki kandungan saja (20,1%).
منابع مشابه
Effects of difructose anhydride III (DFA III) administration on rat intestinal microbiota.
The effects of difructose anhydride III (di-D-fructofuranose-1,2':2,3'-dianhydride; DFA III) administration (3% DFA III for 4 weeks) on rat intestinal microbiota were examined using denaturing gradient gel electrophoresis (DGGE). According to DGGE profiles, the number of bacteria related to Bacteroides acidofaciens and uncultured bacteria within the Clostridium lituseburense group decreased, wh...
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The growth of DFA III-assimilating bacteria in the intestines of rats fed 3% DFA III for 2 weeks was examined. Sixty-four percent of the DFA III intake had been assimilated on day 3 of ingestion, and almost all of the DFA III was assimilated at the end of the experiment. The DFA III-assimilating bacterium, Ruminococcus productus, in DFA III-fed rats was in the stationary state of 10(8)-10(9) ce...
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Changes in the intestinal microbiota of 10 human subjects with long-term ingestion of 3 g/d difructose anhydride III (DFA III; 4 persons, 2 months; 3 persons, 6 months; and 3 persons, 12 months) were examined by denaturing gradient gel electrophoresis (DGGE). According to the answers to questionnaires, the subjects were divided into two groups (constipated and normal). The DGGE profile was diff...
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ژورنال
عنوان ژورنال: Agritech
سال: 2022
ISSN: ['0216-0455', '2527-3825']
DOI: https://doi.org/10.22146/agritech.68463